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Imaging of Developing Metaxylem Vessel Elements in Cultured Hypocotyls

  • Takema Sasaki
  • Yoshihisa OdaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1992)

Abstract

An in vitro induction system for xylem vessel formation is a useful tool for visualizing the differentiation of xylem vessel cells. A procedure for inducing xylem vessel cell differentiation in hypocotyls of Arabidopsis thaliana is described here. Metaxylem vessel elements form ectopically in excised hypocotyl tissue following treatment with bikinin. This enables high-resolution imaging of living metaxylem vessel cells. The wide range of resources available for Arabidopsis allows for the visualization of diverse cellular structures, including microtubules and secondary cell walls, in different genetic backgrounds. Use of this system will contribute to the further understanding of the processes by which xylem vessel elements form.

Key words

Bikinin Cell wall Microtubule Tracheary element Xylem 

Notes

Acknowledgments

This work was supported by grants from JSPS KAKENHI (18H02469 to Y.O. and 18 K14737 to T.S.).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Center for Frontier ResearchNational Institute of GeneticsMishimaJapan
  2. 2.Department of GeneticsSOKENDAI (Graduate University for Advanced Studies)MishimaJapan

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