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The Photoconvertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics

  • Alexandra Lešková
  • Zuzana Kusá
  • Mária Labajová
  • Miroslav Krausko
  • Ján JásikEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1992)

Abstract

Fluorescence proteins changing spectral properties after exposure to light with a specific wavelength have recently become outstanding aids in the study of intracellular protein dynamics. Herein we show using Arabidopsis SYNAPTOTAGMIN 1 as a model protein that the Dendra2 green to red photoconvertible protein tag in combination with confocal scanning laser microscopy is a useful tool to study membrane protein intracellular dynamics.

Key words

Photoconvertible fluorescence proteins Dendra2 Photoactivated localization microscopy Arabidopsis SYT1 

Notes

Acknowledgments

This work has been supported by the Slovak Research and Development Agency (grant no. APVV-16-0398). J.J. thanks also the Alexander von Humboldt Foundation for supporting his renewed research stay at MPIPZ, Cologne.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Alexandra Lešková
    • 1
  • Zuzana Kusá
    • 1
  • Mária Labajová
    • 2
  • Miroslav Krausko
    • 1
  • Ján Jásik
    • 1
    Email author
  1. 1.Plant Science and Biodiversity Center, Institute of BotanySlovak Academy of SciencesBratislavaSlovakia
  2. 2.Plant Science and Biodiversity Center, Institute of Botany of the Czech Academy of SciencesSlovak Academy of SciencesBratislavaSlovakia

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