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A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance

  • Eduardo Balsanelli
  • Vânia Carla Pankievicz
  • Valter Antonio Baura
  • Fábio de Oliveira Pedrosa
  • Emanuel Maltempi de SouzaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1991)

Abstract

The crescent knowledge in the microbiology field guided by microbiome studies has renewed interest in microbes that interact with the plant host in a beneficial manner, especially those that live in the rhizosphere or inside plant tissues and promote plant growth by various mechanisms. Here a comprehensive method for efficient isolation of beneficial endophytes from sterile and open systems, and selection of effective graminaceous plant root colonizers by competitive interactions with plants is described. Parameters for plant cultivation and bacterial inoculation to assess plant growth promotion using the plant model S. viridis are described as components of this method.

Key words

Plant growth promotion BNF Endophytes Model plant Beneficial inoculation PGPR isolation “H” tube system 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Eduardo Balsanelli
    • 1
  • Vânia Carla Pankievicz
    • 1
  • Valter Antonio Baura
    • 1
  • Fábio de Oliveira Pedrosa
    • 1
  • Emanuel Maltempi de Souza
    • 1
    Email author
  1. 1.Department of Biochemistry and Molecular BiologyFederal University of ParanáCuritibaBrazil

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