A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance
The crescent knowledge in the microbiology field guided by microbiome studies has renewed interest in microbes that interact with the plant host in a beneficial manner, especially those that live in the rhizosphere or inside plant tissues and promote plant growth by various mechanisms. Here a comprehensive method for efficient isolation of beneficial endophytes from sterile and open systems, and selection of effective graminaceous plant root colonizers by competitive interactions with plants is described. Parameters for plant cultivation and bacterial inoculation to assess plant growth promotion using the plant model S. viridis are described as components of this method.
Key wordsPlant growth promotion BNF Endophytes Model plant Beneficial inoculation PGPR isolation “H” tube system
- 3.Souza EM, Pedrosa FO (2015) Inorganic Nitrogen Metabolism in Azospirillum spp. In: Cassán FD, Okon Y, Creus CM (eds) Handbook for Azospirillum, Technical Issues and Protocols. Springer International Publishing Switzerland, SpringerGoogle Scholar
- 5.Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Berkeley, CaliforniaGoogle Scholar
- 7.Magalhães Cruz L, Souza EM et al (2001) 16S ribosomal DNA characterization of nitrogen-fixing bacteria isolated from banana (Musa spp.) and pineapple (Ananas comosus (L.) Merril). Appl Environ Microbiol 67:2375–2379. https://doi.org/10.1128/AEM.67.5.2375-2379.2001CrossRefPubMedPubMedCentralGoogle Scholar