A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance

  • Eduardo Balsanelli
  • Vânia Carla Pankievicz
  • Valter Antonio Baura
  • Fábio de Oliveira Pedrosa
  • Emanuel Maltempi de SouzaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1991)


The crescent knowledge in the microbiology field guided by microbiome studies has renewed interest in microbes that interact with the plant host in a beneficial manner, especially those that live in the rhizosphere or inside plant tissues and promote plant growth by various mechanisms. Here a comprehensive method for efficient isolation of beneficial endophytes from sterile and open systems, and selection of effective graminaceous plant root colonizers by competitive interactions with plants is described. Parameters for plant cultivation and bacterial inoculation to assess plant growth promotion using the plant model S. viridis are described as components of this method.

Key words

Plant growth promotion BNF Endophytes Model plant Beneficial inoculation PGPR isolation “H” tube system 


  1. 1.
    Pankievicz VCS, do Amaral FP et al (2015) Robust biological nitrogen fixation in a model grass-bacterial association. Plant J 81:907–919. Scholar
  2. 2.
    Baldani I, Reis VM et al (2014) The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiologists. Plant and Soil 384:413–431. Scholar
  3. 3.
    Souza EM, Pedrosa FO (2015) Inorganic Nitrogen Metabolism in Azospirillum spp. In: Cassán FD, Okon Y, Creus CM (eds) Handbook for Azospirillum, Technical Issues and Protocols. Springer International Publishing Switzerland, SpringerGoogle Scholar
  4. 4.
    Machado HB, Funayama S et al (1991) Excretion of ammonium by Azospirillum brasilense mutants resistant to ethylenediamine. Can J Microbiol 37:549–555CrossRefGoogle Scholar
  5. 5.
    Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Berkeley, CaliforniaGoogle Scholar
  6. 6.
    Beneduzi A, Peres D, da Costa PB (2008) Genetic and phenotypic diversity of plant-growth-promoting bacilli isolated from wheat fields in southern Brazil. Res Microbiol 159:244–250. Scholar
  7. 7.
    Magalhães Cruz L, Souza EM et al (2001) 16S ribosomal DNA characterization of nitrogen-fixing bacteria isolated from banana (Musa spp.) and pineapple (Ananas comosus (L.) Merril). Appl Environ Microbiol 67:2375–2379. Scholar
  8. 8.
    Altschul SF, Gish W et al (1990) Basic local alignment search tool. J Mol Biol 215:403–410. Scholar
  9. 9.
    Balsanelli E, Tuleski TR, Baura VA et al (2013) Maize root lectins mediate the interaction with Herbaspirillum seropedicae via N-acetyl glucosamine residues of lipopolysaccharides. PLoS One 8:e77001. Scholar
  10. 10.
    Martins PK, Nakayama TJ, Ribeiro AP et al (2015) Floral-dip: a simple and rapid. Biotechnol Rep (Amst) 6:61–63. Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Eduardo Balsanelli
    • 1
  • Vânia Carla Pankievicz
    • 1
  • Valter Antonio Baura
    • 1
  • Fábio de Oliveira Pedrosa
    • 1
  • Emanuel Maltempi de Souza
    • 1
    Email author
  1. 1.Department of Biochemistry and Molecular BiologyFederal University of ParanáCuritibaBrazil

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