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Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant Growth

  • Robert J. KremerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1991)

Abstract

Sampling and handling of soils and rhizosphere soil are very critical steps for obtaining representative microbial cultures and genomic material from these environments. Attention to position in the landscape of a sampling site, previous management of the site, time of year, and depth of sampling is important to assure representative samples. Detailed protocols are provided to assist environmental microbiologists and molecular biologists in proper sampling and handling of soils that serve as the source of cultures and DNA for subsequent use in important experiments carried out in the laboratory.

Key words

Soil profile Landscape position Composite soil sample Soil microbial community Soil survey Rhizosphere 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Plant SciencesUniversity of MissouriColumbiaUSA

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