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Acquisition, Processing, and Quality Control of Mass Cytometry Data

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Mass Cytometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1989))

Abstract

Mass cytometry uniquely combines the principles of mass spectrometry and flow cytometry for high dimensional profiling of immune cells at a single cell level. Using isotopically conjugated antibodies, mass cytometry overcomes the limitations of spectral overlap associated with flow cytometry and allows for deeper single cell characterization of complex biospecimens using more cellular markers. However, the nature of mass spectrometry-based single cell measurements requires specific considerations in acquiring and processing data. This chapter provides an overview of how to optimally acquire mass cytometry data and how to process this data for subsequent analysis and characterization of cell populations.

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Author information

Authors and Affiliations

  1. Human Immune Monitoring Center, Icahn School of Medicine at Mt. Sinai, New York, NY, USA

    Brian H. Lee & Adeeb H. Rahman

  2. Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mt. Sinai, New York, NY, USA

    Adeeb H. Rahman

Authors
  1. Brian H. Lee
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  2. Adeeb H. Rahman
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Corresponding author

Correspondence to Adeeb H. Rahman .

Editor information

Editors and Affiliations

  1. Discipline of Pathology, The University of Sydney, Camperdown, NSW, Australia

    Helen M. McGuire

  2. Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Camperdown, NSW, Australia

    Thomas M. Ashhurst

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Lee, B.H., Rahman, A.H. (2019). Acquisition, Processing, and Quality Control of Mass Cytometry Data. In: McGuire, H., Ashhurst, T. (eds) Mass Cytometry. Methods in Molecular Biology, vol 1989. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9454-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-9454-0_2

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9453-3

  • Online ISBN: 978-1-4939-9454-0

  • eBook Packages: Springer Protocols

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