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Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis

  • Sanne Van Campenhout
  • Hans Van Vlierberghe
  • Lindsey DevisscherEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1981)

Abstract

Cholestatic liver disease covers a range of biliary disorders marked by an impaired bile duct flow. Various conditions can result in bile obstruction including choledocholithiasis, surgical trauma, and autoimmune disorders. Cholestatic liver disease can be mild but generally progresses to more severe conditions with increased hepatobiliary injury, cholangitis, and ultimately liver fibrosis and cirrhosis. An extensively used experimental model to investigate the pathophysiology of biliary cirrhosis and potential novel therapies is the common bile duct ligation in mice and rats. Common bile duct ligation induces the different stages of cholestatic-induced liver disease being cholestasis, subsequently accompanied by inflammation and finally liver fibrosis and cirrhosis. In this protocol, an outline of the surgical procedures to conduct common bile duct ligation in mice is provided. The major steps include the isolation of the common bile duct, followed by ligation and dissection.

Key words

Common bile duct ligation Cholestasis Biliary cirrhosis Liver disease Mouse surgery Portal hypertension Hepatopulmonary syndrome Obstructive jaundice Animal model Ascites 

Notes

Acknowledgments

This work was financially supported by the grants of the Special Research Funds—University Ghent (BOF-UGent) and the Fund for Scientific Research—Flanders (FWO-Vlaanderen).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Sanne Van Campenhout
    • 1
  • Hans Van Vlierberghe
    • 1
  • Lindsey Devisscher
    • 2
    Email author
  1. 1.Internal Medicine and PediatricsGhent UniversityGhentBelgium
  2. 2.Basic and Applied Medical SciencesGhent UniversityGhentBelgium

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