Technologies for Live Imaging of Enteric Neural Crest-Derived Cells

  • Marlene M. Hao
  • Annette J. Bergner
  • Donald F. Newgreen
  • Hideki Enomoto
  • Heather M. Young
Part of the Methods in Molecular Biology book series (MIMB, volume 1976)


Time-lapse imaging of gut explants from embryonic mice in which neural crest-derived cells express fluorescent proteins allows the behavior of enteric neural crest cells to be observed and analyzed. Explants of embryonic gut are dissected, mounted on filter paper supports so the gut retains its tubular three-dimensional structure, and then placed in coverglass bottom culture dishes in tissue culture medium. A stainless steel ring is placed on top of the filter support to prevent movement. Imaging is performed using a confocal microscope in an environmental chamber. A z series of images through the network of fluorescent cells is collected every 3, 5, or 10 min. At the end of imaging, the z series are projected.

Key words

Time-lapse imaging Live imaging Enteric nervous system Cell migration Vagal neural crest 



This work was supported by the Australian Research Council Discovery Grant DP150103709 to H.M.Y., National Health and Medical Research Council (NHMRC) Training Fellowship APP1071153 to M.M.H., and NHMRC Senior Research Fellowship APP1103297 to H.M.Y.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Marlene M. Hao
    • 1
    • 2
  • Annette J. Bergner
    • 1
  • Donald F. Newgreen
    • 3
  • Hideki Enomoto
    • 4
    • 5
  • Heather M. Young
    • 1
  1. 1.Department of Anatomy and NeuroscienceUniversity of MelbourneMelbourneAustralia
  2. 2.Laboratory for Enteric Neuroscience, TARGIDUniversity of LeuvenLeuvenBelgium
  3. 3.Murdoch Children’s Research InstituteRoyal Children’s HospitalParkvilleAustralia
  4. 4.Division of Neural Differentiation and Regeneration, Department of Physiology and Cell Biology, Graduate School of MedicineKobe UniversityKobeJapan
  5. 5.Core Research for Evolutional Science and TechnologyJapan Science and Technology AgencySaitamaJapan

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