Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells

  • Kwaku Dad Abu-Bonsrah
  • Serena Viventi
  • Donald F. Newgreen
  • Mirella DottoriEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1976)


There are a vast range of diseases and disorders that are neurocristopathic in origin, including Hirschsprung’s disease, pheochromocytoma, familial dysautonomia, craniofacial disorders, and melanomas. Having a source of human neural crest cells is highly valuable for investigating potential treatments for such diseases. This chapter describes a robust and well-characterized protocol for deriving neural crest from human pluripotent stem cells (hPSCs), which can then be differentiated to neuronal and non-neuronal lineages. The protocol is adapted to suit hPSC maintenance as a monolayer bulk culture or as manual-passaged colonies, which makes it widely applicable to researchers that may use different systems for hPSC maintenance.

Key words

Neural crest Human pluripotent stem cells Neurons Neural crest differentiation 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kwaku Dad Abu-Bonsrah
    • 1
    • 2
  • Serena Viventi
    • 3
  • Donald F. Newgreen
    • 2
  • Mirella Dottori
    • 3
    • 4
    Email author
  1. 1.Department of PaedriatricsUniversity of MelbourneParkvilleAustralia
  2. 2.Murdoch Childrens Research InstituteParkvilleAustralia
  3. 3.Department of Biomedical Engineering, Centre for Neural EngineeringUniversity of MelbourneParkvilleAustralia
  4. 4.Illawarra Health and Medical Research InstituteUniversity of WollongongWollongongAustralia

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