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In Vivo and In Vitro Quantitative Analysis of Neural Crest Cell Migration

  • Elias H. Barriga
  • Adam Shellard
  • Roberto MayorEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1976)

Abstract

The neural crest is an embryonic cell population induced at the border of the neural plate from where it delaminates and migrates long distances across the embryo. Due to its extraordinary migratory capabilities, the neural crest has become a powerful system to study cellular and molecular aspects of collective and single cell migration both in vivo and in vitro. Here we provide detailed protocols used to perform quantitative analysis of molecular and cellular aspects of Xenopus laevis neural crest cell migration, both in vivo and in vitro.

Key words

Neural crest cells Quantitative migration In situ hybridization Xenopus laevis Cell migration Cell dispersion Grafts Delaunay-Voronoi triangulation Neural crest dissection 

Notes

Acknowledgments

This study was supported by grants from MRC (M010465 and J000655) and BBSRC (M008517) and Wellcome Trust to R.M., an EMBO Long-Term Fellowship and a Marie Curie Fellowship (329968) to E.H.B., and a Wellcome Trust fellowship to A.S.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Elias H. Barriga
    • 1
    • 2
  • Adam Shellard
    • 1
  • Roberto Mayor
    • 1
    Email author
  1. 1.Department of Cell and Developmental BiologyUniversity College LondonLondonUK
  2. 2.London Centre for NanotechnologyUniversity College LondonLondonUK

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