Abstract
Seq-Well is a low-cost picowell platform that can be used to simultaneously profile the transcriptomes of thousands of cells from diverse, low input clinical samples. In Seq-Well, uniquely barcoded mRNA capture beads and cells are co-confined in picowells that are sealed using a semipermeable membrane, enabling efficient cell lysis and mRNA capture. The beads are subsequently removed and processed in parallel for sequencing, with each transcript’s cell of origin determined via the unique barcodes. Due to its simplicity and portability, Seq-Well can be performed almost anywhere.
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Acknowledgments
R.G. was supported by the Intramural Research Program of the Division of Intramural Research Z01AI000947, NIAID, NIH; the UCLA-Caltech MSTP, and the NIGMS T32 GM008042. A.K.S. was supported by the Searle Scholars Program, the Beckman Young Investigator Program, the Pew-Stewart Scholars, a Sloan Fellowship in Chemistry, NIH grants 1DP2OD020839, 2U19AI089992, 1U54CA217377, P01AI039671, 5U24AI118672, 2RM1HG006193, 1R33CA202820, 2R01HL095791, 1R01AI138546, 1R01HL126554, 1R01DA046277, 2R01HL095791, and Bill and Melinda Gates Foundation grants OPP1139972, OPP1137006, and OPP1116944. J.C.L. was supported by NIH grants DP3DK09768101, P01AI045757, R21AI106025, and R56AI104274, the W.M. Keck Foundation, Camille Dreyfus Teacher-Scholar program, and the US Army Research Office through the Institute for Soldier Nanotechnologies, under contract number W911NF-13-D-0001. This work was also supported in part by the Koch Institute Support (core) NIH Grant P30-CA14051 from the National Cancer Institute.
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Aicher, T.P. et al. (2019). Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA Sequencing. In: Proserpio, V. (eds) Single Cell Methods. Methods in Molecular Biology, vol 1979. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9240-9_8
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DOI: https://doi.org/10.1007/978-1-4939-9240-9_8
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