Synthesis of Base-Modified dNTPs Through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA
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Synthesis of base-modified dNTPs through the Suzuki or Sonogashira cross-coupling reactions of halogenated dNTPs with boronic acids or alkynes is reported, as well as the use of these modified dNTPs in polymerase incorporations to oligonucleotides or DNA by primer extension or PCR.
Key wordsNucleotides Nucleoside triphosphates DNA polymerases Enzymatic synthesis Modified DNA
This work was supported by the Academy of Sciences of the Czech Republic (Praemium Academiae), by the Czech Science Foundation (18-03305S), and by the European Regional Development Fund; OP RDE (No. CZ.02.1.01/0.0/0.0/16_019/0000729).
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