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Synthesis of Base-Modified dNTPs Through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA

  • Petra Ménová
  • Hana Cahová
  • Milan Vrábel
  • Michal HocekEmail author
Protocol
  • 312 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 1973)

Abstract

Synthesis of base-modified dNTPs through the Suzuki or Sonogashira cross-coupling reactions of halogenated dNTPs with boronic acids or alkynes is reported, as well as the use of these modified dNTPs in polymerase incorporations to oligonucleotides or DNA by primer extension or PCR.

Key words

Nucleotides Nucleoside triphosphates DNA polymerases Enzymatic synthesis Modified DNA 

Notes

Acknowledgments

This work was supported by the Academy of Sciences of the Czech Republic (Praemium Academiae), by the Czech Science Foundation (18-03305S), and by the European Regional Development Fund; OP RDE (No. CZ.02.1.01/0.0/0.0/16_019/0000729).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Petra Ménová
    • 1
  • Hana Cahová
    • 1
  • Milan Vrábel
    • 1
  • Michal Hocek
    • 1
    Email author
  1. 1.Institute of Organic Chemistry and BiochemistryCzech Academy of SciencesPragueCzech Republic

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