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Prostate Protein n-Glycosylation Profiling by Means of DNA Sequencer-Assisted Fluorophore-Assisted Carbohydrate Electrophoresis

  • Tijl Vermassen
  • Nico Callewaert
  • Sylvie Rottey
  • Joris R. DelangheEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1972)

Abstract

DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis allows for accurate profiling of the asparagine-linked (n-) glycosylation patterns, a posttranslational modification present on many soluble and membrane proteins. This technique has been extensively tested to identify n-glycosylation patterns associated with serum proteins. Here we describe the use of DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis to identify the n-glycosylation patterns of prostate proteins in urine.

Key words

n-Glycosylation Carbohydrate electrophoresis Capillary electrophoresis Urine Fluorophore 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Tijl Vermassen
    • 1
    • 3
  • Nico Callewaert
    • 2
  • Sylvie Rottey
    • 1
    • 3
  • Joris R. Delanghe
    • 4
    Email author
  1. 1.Department of Medical OncologyGhent University HospitalGhentBelgium
  2. 2.Center for Medical Biotechnology, VIBGhentBelgium
  3. 3.Drug Research Unit GhentGhent University HospitalGhentBelgium
  4. 4.Department of Clinical Chemistry, Microbiology and ImmunologyGhent UniversityGhentBelgium

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