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Capillary Electrophoresis Analysis of Prostate-Specific Antigen (PSA)

  • Noemi Farina-Gomez
  • Diana Navarro-Calderon
  • Angel Puerta
  • Monica Gonzalez
  • José Carlos Diez-Masa
  • Mercedes de FrutosEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1972)

Abstract

The Capillary Electrophoresis (CE) profile of isoforms (peaks) of a glycoprotein can be useful to show alterations in its posttranslational modifications (PTMs) linked to diseases. These changes can modify the electrophoretic mobility of these isoforms in a minor extent and, therefore, very reproducible CE methods are needed to detect them. In this chapter, a method for the analysis of prostate-specific antigen (PSA) by Capillary Zone Electrophoresis (CZE) with UV detection is detailed. High reproducibility in the separation of a large number of PSA isoforms is achieved by performing capillary conditioning in acid media and by using a background electrolyte (BGE) at pH 8.0 formulated with decamethonium bromide and urea.

Key words

Reproducibility Capillary conditioning Prostate-specific antigen PSA Isoform Proteoform Glycoform Cancer biomarker 

Notes

Acknowledgments

Financial support from the Spanish Ministry of Economy and Competitiveness (project CTQ2013-43236-R) is acknowledged. Noemi Farina-Gomez and Monica Gonzalez acknowledge the Ph.D. JAE-pre grant and the JAE-doc grant, respectively, from CSIC, co-financed by the European Social Fund (ESF). Diana Navarro-Calderon acknowledges her contract in the frame of the Youth Guarantee Implementation Plans, financed by the ESF and the Youth Employment Initiative (YEI).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Noemi Farina-Gomez
    • 1
  • Diana Navarro-Calderon
    • 1
  • Angel Puerta
    • 1
  • Monica Gonzalez
    • 1
  • José Carlos Diez-Masa
    • 1
  • Mercedes de Frutos
    • 1
    Email author
  1. 1.Institute of Organic Chemistry, (IQOG-CSIC)MadridSpain

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