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Leishmania pp 351-368 | Cite as

Establishment, Maintenance of Phlebotomus spp. in the Laboratory, and Infection with Leishmania spp.

  • Ifhem Chelbi
  • Elyes Zhioua
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1971)

Abstract

Sand fly colonies are of major importance for experimental studies on biology, behavior, vector competence, relationship with Leishmania parasites, and vector control. This chapter is intended to provide methods and techniques used to initiate, establish, and maintain sand fly colonies. Details on collecting sand flies for colonization, colony initiation, maintenance, and experimental infection of Phlebotomus spp. with Leishmania spp. are reported.

Key words

Phlebotomus Colonization Leishmania infection 

References

  1. 1.
    Safyanova VM (1964) Laboratory cultivation of sandflies (Diptera: Phlebotominae). Bull WHO 31:573–576PubMedGoogle Scholar
  2. 2.
    Endris RG, Perkins PV, Young DG, Johnson RD (1982) Techniques for laboratory rearing of sand flies (Diptera: Psychodidae). Mosquito News 42:400–407Google Scholar
  3. 3.
    Killick-kendrick R, Leaney AJ, Ready PD (1977) The establishment, maintenance and productivity of a laboratory colony of Lutzomyia longipalpis (Diptera: Psychodidae). J Med Entomol 13:429–440CrossRefGoogle Scholar
  4. 4.
    Lawyer P, Killick-Kendrick M, Rowland T, Rawton E, Volf P (2017) Laboratory colonization and mass rearing of phlebotomine sand flies (Diptera: Psychodidae). Parasite 24:42.  https://doi.org/10.1051/parasite/2017041CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Valenzuela J, Belkaid Y, Garfiela MK, Mendez S, Kamhawi S, Rowton E, Sacks D, Ribeiro JMC (2001) Toward a defined anti-Leishmania vaccine targeting vector antigens: characterization of a protective salivary protein. J Exp Med 194:331–342CrossRefGoogle Scholar
  6. 6.
    Anonymous (1977) Report of the first meeting, Scientific Working Group on leishmaniasis. World Health Organization Special Program for Research and Training in Tropical Diseases, Geneva, p 31Google Scholar
  7. 7.
    Chelbi I, Zhioua E (2007) Biology of Phlebotomus papatasi Scopoli (Diptera: Psychodidae) in the laboratory. J Med Entomol 44:597–600CrossRefGoogle Scholar
  8. 8.
    Lawyer PG, Rowton ED, Perkins PV, Johnson RN, Young DG (1991) Recent advances in laboratory mass rearing of phlebotomine sand flies. Parassitologia 33(Suppl. 1):361–364PubMedGoogle Scholar
  9. 9.
    Maroli M, Fiorentino S, Guandalini E (1987) Biology of a laboratory colony of Phlebotomus perniciosus (Diptera: Psychodidae). J Med Entomol 24:547–551CrossRefGoogle Scholar
  10. 10.
    Modi GB, Tesh RB (1983) A simple technique for mass rearing Lutzomyia longipalpis and Phlebotomus papatasi (Diptera: Psychodidae) in the laboratory. J Med Entomol 20:568–569CrossRefGoogle Scholar
  11. 11.
    Ward RD (1977) The colonization of Lutzomyia flaviscutellata (Diptera: Psychodidae), a vector of Leishmania mexicana amazonensis in Brazil. J Med Entomol 14:469–476CrossRefGoogle Scholar
  12. 12.
    Croset H, Rioux JA, Master M, Bayar N (1978) Les phlébotomes de la Tunisie (Diptera, Phlebotominae). Mise au point systématique, chorologique et éthologique. Ann Parasitol Hum Comp 53:711–749CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ifhem Chelbi
    • 1
  • Elyes Zhioua
    • 1
  1. 1.Unit of Vector EcologyInstitut Pasteur de TunisTunisTunisia

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