The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp.

  • Emily A. Kibble
  • Mitali Sarkar-Tyson
  • Geoffrey W. Coombs
  • Charlene M. KahlerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1969)


Neisseria meningitidis and Neisseria gonorrhoeae are obligate pathogens of the human host. Due to their adaptation to the human host, many factors required for infection are specialized for the human host to the point that natural infection processes are difficult to replicate in animal models. Immortalized human cell lines have been used to identify the host factors necessary for successful colonization of human mucosal surfaces. One such model is the Detroit 562 pharyngeal immortalized cell monolayer model which is used to measure the rate of attachment to and invasion of N. meningitidis and N. gonorrhoeae into epithelial cells. The methodology of this assay, as well as the maintenance of Detroit 562 cells necessary for the experiment, will be described.

Key words

Detroit 562 Pharyngeal Meningococcal Gonococcal Host receptors 



This work is supported by the Australian Defence Material Transfer Center (Project Agreement 10.44) and North Atlantic Treaty Organization (SfP-984235) awarded to MST. EK is supported by a Research Training Program (RTP) PhD scholarship from Murdoch University. CK is supported by the Amanda Young Foundation, a not-for-profit organisation, for improved community awareness of meningococcal disease.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Emily A. Kibble
    • 1
    • 2
  • Mitali Sarkar-Tyson
    • 1
  • Geoffrey W. Coombs
    • 2
  • Charlene M. Kahler
    • 1
    Email author
  1. 1.Marshall Centre for Infectious Diseases Research and Training, School of Biomedical SciencesUniversity of Western AustraliaPerthAustralia
  2. 2.School of Veterinary and Life SciencesMurdoch UniversityPerthAustralia

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