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Sample Preparation for Proteomic Analysis of Neisseria meningitidis

  • Benjamin L. SchulzEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1969)

Abstract

Mass spectrometry (MS) proteomics allows systematic identification, characterization, and relative quantification of the full suite of proteins in a biological sample, and is a powerful analytical approach for investigation of many aspects of the biology of Neisseria meningitidis. Here, we describe methods for robust and efficient sample preparation of the proteome of N. meningitidis suitable for diverse MS proteomics workflows.

Key words

Mass spectrometry Bottom-up proteomics Sample preparation 

Notes

Acknowledgments

This work was supported by National Health and Medical Research Council Career Development Fellowship APP1087975 to B.L.S.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Chemistry and Molecular BiosciencesThe University of QueenslandBrisbaneAustralia
  2. 2.Australian Infectious Diseases Research CentreThe University of QueenslandBrisbaneAustralia

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