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Isolation and Characterization of Adipose Tissue Macrophages

  • Grace Ampem
  • Tamás RöszerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1966)

Abstract

This chapter describes a technique that can be used to isolate adipose tissue macrophages (ATMs) from the visceral white adipose tissue. Nevertheless, this technique can also be used to isolate ATMs from subcutaneous white adipose tissue and brown adipose tissue from mouse, human subcutaneous fat depot, and also from the fat body of the toad Xenopus. We detail the flow-cytometric gating strategy that has been developed to identify ATM population, and we describe the isolation of RNA from this population and its use for gene expression profiling. Finally, we describe in vitro culture of ATMs for downstream applications.

Key words

Macrophage Adipose tissue Flow cytometry (FACS) Immunity Ribonucleic acid (RNA) 

Notes

Acknowledgments

The work in the authors’ laboratory is supported by a German Research Fund (DFG) grant and by the European Foundation for the Study of Diabetes (EFSD). Scopus author identifiers of the authors are 56764443800 (G.A.) and 7801656864 (T.R.).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Comparative Molecular Endocrinology, Centre for Biomedical ResearchUniversity of UlmUlmGermany
  2. 2.Institute of NeurobiologyUniversity of UlmUlmGermany

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