Abstract
The chemical carcinogen 7,12-dimethylbenz[a]anthracene (DMBA) has been used for many decades to induce skin, mammary, and ovarian tumors in mice. There are however a wide range of doses and treatment regimens in the literature that sometimes confound comparative interpretations of different studies. Here we describe a proven method to generate in vivo DMBA-mediated murine mammary tumors to enable consistent studies of the cell targeted role of genes of interest during this process.
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Acknowledgments
We wish to thank Queen’s Quality Assurance and Training Coordinator, Erin Burnett, for providing photos for submandibular bleed and oral gavage procedures, and the Queen’s University Veterinarian, Dr. Andrew Winterborn, for his permission to use the images.
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Shi, J.Y., Rubino, R.E., Nicol, C.J.B. (2019). Examining the Role of Nuclear Receptors During In Vivo Chemical-Mediated Breast Tumorigenesis. In: Badr, M. (eds) Nuclear Receptors. Methods in Molecular Biology, vol 1966. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9195-2_16
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DOI: https://doi.org/10.1007/978-1-4939-9195-2_16
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