Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry
This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (ΔrH), Km, kcat, and vmax are determined in a single assay that does not require labeling or immobilization.
Key wordsKinetics ITC Michaelis-Menten Enzyme Single-injection method Calorimetry Enthalpy
- 4.Choma CT (2006). Characterizing enzyme kinetics by ITC. Applications Note M125, TA Instruments. http://www.tainstruments.com. Accessed 06 Sept 2012