Ancient DNA pp 65-73 | Cite as

Double-Stranded Library Preparation for Ancient and Other Degraded Samples

  • Kirstin Henneberger
  • Axel Barlow
  • Johanna L. A. Paijmans
Part of the Methods in Molecular Biology book series (MIMB, volume 1963)


High-throughput sequencing (HTS) allows fast and cost-efficient sequencing of ancient DNA (aDNA) without prior information about what sequences should be targeted. One necessary step for HTS is the preparation of a sequencing library. Commercial kits are available for this purpose, but many of these are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.

Key words

Double-stranded library preparation Degraded DNA Ancient DNA Historical DNA Illumina sequencing 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kirstin Henneberger
    • 1
  • Axel Barlow
    • 1
  • Johanna L. A. Paijmans
    • 1
  1. 1.Institute for Biochemistry and BiologyUniversity of PotsdamPotsdamGermany

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