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Influenza-Mediated Lung Infection Models

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Mouse Models of Innate Immunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1960))

Abstract

Laboratory rodent influenza infection models have been and continue to be a critical tool for understanding virus-host interactions during infection. The incidence of seasonal influenza infections coupled with the need for novel therapeutics and universal vaccines highlights the need to uncover novel mechanisms of pathogenesis and protection. Mouse models are extremely useful for the evaluation of influenza vaccines and provide an invaluable tool to probe the immune response. This chapter describes the technique of intranasal inoculation of male C57BL/6J mice with an H1N1 strain of influenza (A/Puerto Rico/8/1934) and methods for assessing the optimum dose for infection, viral titers in lung tissue, and severity of disease.

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References

  1. Flannery B, Chung JR, Thaker SN et al (2017) Interim estimates of 2016-17 seasonal influenza vaccine effectiveness - United States, February 2017. MMWR Morb Mortal Wkly Rep 66(6):167–171. https://doi.org/10.15585/mmwr.mm6606a3

    Article  PubMed  PubMed Central  Google Scholar 

  2. Dugan VG, Blanton L, Elal AIA et al (2017) Update: influenza activity - United States, October 1-November 25, 2017. MMWR Morb Mortal Wkly Rep 66(48):1318–1326. https://doi.org/10.15585/mmwr.mm6648a2

    Article  PubMed  PubMed Central  Google Scholar 

  3. Blanton L, Alabi N, Mustaquim D et al (2017) Update: influenza activity in the United States during the 2016-17 season and composition of the 2017-18 influenza vaccine. MMWR Morb Mortal Wkly Rep 66(25):668–676. https://doi.org/10.15585/mmwr.mm6625a3

    Article  PubMed  PubMed Central  Google Scholar 

  4. Eilers R, Krabbe PF, van Essen TG et al (2013) Assessment of vaccine candidates for persons aged 50 and older: a review. BMC Geriatr 13:32. https://doi.org/10.1186/1471-2318-13-32

    Article  PubMed  PubMed Central  Google Scholar 

  5. Powell ND, Allen RG, Hufnagle AR et al (2011) Stressor-induced alterations of adaptive immunity to vaccination and viral pathogens. Immunol Allergy Clin N Am 31(1):69–79. https://doi.org/10.1016/j.iac.2010.09.002

    Article  Google Scholar 

  6. Dutkowski R (2010) Oseltamivir in seasonal influenza: cumulative experience in low- and high-risk patients. J Antimicrob Chemother 65(Suppl 2):ii11–ii24. https://doi.org/10.1093/jac/dkq012

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Erbelding EJ, Post D, Stemmy E et al (2018) A universal influenza vaccine: the strategic plan for the National Institute of Allergy and Infectious Diseases. J Infect Dis. https://doi.org/10.1093/infdis/jiy103

    Article  Google Scholar 

  8. Thangavel RR, Bouvier NM (2014) Animal models for influenza virus pathogenesis, transmission, and immunology. J Immunol Methods 410:60–79. https://doi.org/10.1016/j.jim.2014.03.023

    Article  CAS  PubMed  Google Scholar 

  9. Trabalon M, Schaal B (2012) It takes a mouth to eat and a nose to breathe: abnormal oral respiration affects neonates' oral competence and systemic adaptation. Int J Pediatr 2012:207605. https://doi.org/10.1155/2012/207605

    Article  PubMed  PubMed Central  Google Scholar 

  10. Winkler-Heil R, Hofmann W (2016) Modeling particle deposition in the Balb/c mouse respiratory tract. Inhal Toxicol 28(4):180–191. https://doi.org/10.3109/08958378.2016.1148801

    Article  CAS  PubMed  Google Scholar 

  11. Hogan BL, Barkauskas CE, Chapman HA et al (2014) Repair and regeneration of the respiratory system: complexity, plasticity, and mechanisms of lung stem cell function. Cell Stem Cell 15(2):123–138. https://doi.org/10.1016/j.stem.2014.07.012

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Bouvier NM, Lowen AC (2010) Animal models for influenza virus pathogenesis and transmission. Viruses 2(8):1530–1563. https://doi.org/10.3390/v20801530

    Article  PubMed  PubMed Central  Google Scholar 

  13. Trammell RA, Toth LA (2008) Genetic susceptibility and resistance to influenza infection and disease in humans and mice. Expert Rev Mol Diagn 8(4):515–529. https://doi.org/10.1586/14737159.8.4.515

    Article  CAS  PubMed  Google Scholar 

  14. Srivastava B, Blazejewska P, Hessmann M et al (2009) Host genetic background strongly influences the response to influenza a virus infections. PLoS One 4(3):e4857. https://doi.org/10.1371/journal.pone.0004857

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Gazit R, Gruda R, Elboim M et al (2006) Lethal influenza infection in the absence of the natural killer cell receptor gene Ncr1. Nat Immunol 7(5):517–523. https://doi.org/10.1038/ni1322

    Article  CAS  PubMed  Google Scholar 

  16. Bergmann M, Garcia-Sastre A, Carnero E et al (2000) Influenza virus NS1 protein counteracts PKR-mediated inhibition of replication. J Virol 74(13):6203–6206

    Article  CAS  Google Scholar 

  17. Haller O, Arnheiter H, Lindenmann J (1976) Genetically determined resistance to infection by hepatotropic influenza A virus in mice: effect of immunosuppression. Infect Immun 13(3):844–854

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Koerner I, Kochs G, Kalinke U et al (2007) Protective role of beta interferon in host defense against influenza A virus. J Virol 81(4):2025–2030. https://doi.org/10.1128/JVI.01718-06

    Article  CAS  PubMed  Google Scholar 

  19. Staeheli P, Grob R, Meier E et al (1988) Influenza virus-susceptible mice carry Mx genes with a large deletion or a nonsense mutation. Mol Cell Biol 8(10):4518–4523

    Article  CAS  Google Scholar 

  20. Staeheli P, Haller O, Boll W et al (1986) Mx protein: constitutive expression in 3T3 cells transformed with cloned Mx cDNA confers selective resistance to influenza virus. Cell 44(1):147–158

    Article  CAS  Google Scholar 

  21. Vanlaere I, Vanderrijst A, Guenet JL et al (2008) Mx1 causes resistance against influenza A viruses in the Mus spretus-derived inbred mouse strain SPRET/Ei. Cytokine 42(1):62–70. https://doi.org/10.1016/j.cyto.2008.01.013

    Article  CAS  PubMed  Google Scholar 

  22. Haller O, Staeheli P, Kochs G (2007) Interferon-induced Mx proteins in antiviral host defense. Biochimie 89(6–7):812–818. https://doi.org/10.1016/j.biochi.2007.04.015

    Article  CAS  PubMed  Google Scholar 

  23. Matrosovich M, Matrosovich T, Garten W et al (2006) New low-viscosity overlay medium for viral plaque assays. Virol J 3:63. https://doi.org/10.1186/1743-422X-3-63

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

This work was supported by the Virology Unit of the Duke Regional Biocontainment Laboratory (RBL) under the directorship of Dr. Gregory D. Sempowski, which received partial support for construction from the National Institutes of Health, National Institute of Allergy and Infectious Diseases (UC6-AI058607). This work was also supported by the National Institutes of Health, National Institute of Environmental Health Sciences (Z01 ES102005 to MBF).

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Authors and Affiliations

  1. Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC, USA

    Charles E. McGee & Christopher J. Sample

  2. Department of Pharmacology and Toxicology, Brody School of Medicine, East Carolina University, Greenville, NC, USA

    Brita Kilburg-Basnyat & Kymberly M. Gowdy

  3. Immunity, Inflammation, and Disease Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA

    Kristin A. Gabor & Michael B. Fessler

Authors
  1. Charles E. McGee
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  2. Christopher J. Sample
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  3. Brita Kilburg-Basnyat
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  4. Kristin A. Gabor
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  5. Michael B. Fessler
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  6. Kymberly M. Gowdy
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Corresponding author

Correspondence to Kymberly M. Gowdy .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences, Virginia-Maryland Regional College, Blacksburg, VA, USA

    Irving C. Allen

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McGee, C.E., Sample, C.J., Kilburg-Basnyat, B., Gabor, K.A., Fessler, M.B., Gowdy, K.M. (2019). Influenza-Mediated Lung Infection Models. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1960. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9167-9_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9167-9_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9166-2

  • Online ISBN: 978-1-4939-9167-9

  • eBook Packages: Springer Protocols

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