Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics

  • Leslie Muller
  • Luc Fornecker
  • Sarah Cianferani
  • Christine CarapitoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1959)


Sample preparation is a key step in proteomics workflows. Tube-gel (TG) is a fast and repeatable sample preparation method that consists in the instantaneous trapping of the sample in a polyacrylamide gel matrix. It takes advantage of in-gel sample preparations by allowing the use of high concentrations of sodium-dodecyl sulfate but avoids the time-consuming step of electrophoresis. Therefore, TG limits the sample handling and is thus particularly suitable for high-throughput quantitative proteomics when large sample numbers have to be processed, as it is often the case in biomarker research and clinical proteomics projects.

Key words

Tube-gel Sample preparation High-throughput Detergent-compatibility Quantitative proteomics 



This work was supported by the French Proteomic Infrastructure (ProFI; ANR-10-INBS-08-03).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Leslie Muller
    • 1
  • Luc Fornecker
    • 1
  • Sarah Cianferani
    • 1
  • Christine Carapito
    • 1
    Email author
  1. 1.Laboratoire de Spectrométrie de Masse Bio-Organique (LSMBO)IPHC, UMR 7178, Université de Strasbourg, CNRSStrasbourgFrance

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