βαβ Super-Secondary Motifs: Sequence, Structural Overview, and Pursuit of Potential Autonomously Folding βαβ Sequences from (β/α)8/TIM Barrels

  • Rajasekhar Varma Kadamuri
  • Shivkumar Sharma Irukuvajjula
  • Ramakrishna VadrevuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1958)


βαβ super-secondary structures constitute the basic building blocks of (β/α)8 class of proteins. Despite the success in designing super-secondary structures, till date, there is not a single example of a natural βαβ sequence known to fold in isolation. In this chapter, to address the finding the “needles” in the haystack scenario, we have combined the sequence preferences and structural features of independent βαβ motifs, dictated by natural selection, with rationally derived parameters from a designed βαβ motif adopting stable fold in solution. Guided by this approach, a set of potential βαβ sequences from (β/α)8/TIM barrels are proposed as likely candidates for autonomously folding based on the assessment of their foldability.

Key words

βαβ motifs Super-secondary structures Main-chain to side-chain hydrogen bonding (β/α)8 barrels TIM barrel 



The authors thank University Grants Commission, India, for the initial part of this research. RVK is grateful to UGC, India, and Birla Institute of Technology and Science Pilani, Hyderabad Campus, for financial support in the form of research fellowship.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Rajasekhar Varma Kadamuri
    • 1
  • Shivkumar Sharma Irukuvajjula
    • 1
  • Ramakrishna Vadrevu
    • 1
    Email author
  1. 1.Department of Biological SciencesBirla Institute of Technology and Science-PilaniHyderabadIndia

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