Antimicrobial peptides (AMPs) are novel agents for therapeutic application for their inherent broad spectrum of activities against bacteria, fungi, and viruses, as well as anti-inflammatory, anticancerous, and immunomodulatory activities. This chapter presents an enzymatic method to generate glycovariants of one such antimicrobial peptide, namely enterocin 96, using a bacterial protein O- and S-glycosyltransferase, in vitro.
In vitro glycosyltransferase assay Mass spectrometry High performance liquid chromatography (HPLC) Chymotrypsin digestion Exoglycosidase treatment/Deglycosylation assay Glycodiversification
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