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In Vitro Synthesis of Bioactive Glycovariants of Enterocin 96, an Antimicrobial Peptide from Enterococcus faecalis

  • Rupa Nagar
  • Alka RaoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1954)

Abstract

Antimicrobial peptides (AMPs) are novel agents for therapeutic application for their inherent broad spectrum of activities against bacteria, fungi, and viruses, as well as anti-inflammatory, anticancerous, and immunomodulatory activities. This chapter presents an enzymatic method to generate glycovariants of one such antimicrobial peptide, namely enterocin 96, using a bacterial protein O- and S-glycosyltransferase, in vitro.

Key words

In vitro glycosyltransferase assay Mass spectrometry High performance liquid chromatography (HPLC) Chymotrypsin digestion Exoglycosidase treatment/Deglycosylation assay Glycodiversification 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.CSIR-Institute of Microbial Technology (CSIR-IMTECH)ChandigarhIndia

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