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RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-Negative Bacteria

  • Zhongrui Ma
  • Peng George WangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1954)

Abstract

RecET direct cloning enables obtaining a large DNA region from genome. Here we describe its applications in cloning of polysaccharide gene cluster from gram-negative bacteria. Rapid and exact cloning of polysaccharide gene cluster can be achieved by this method.

Key words

RecET direct cloning Polysaccharide gene cluster Gram-negative bacteria Genome isolation Restriction enzyme digestion Linear cloning vector Electroporation 

Notes

Acknowledgments

This work was supported by NIH grant R01AI083754. Thank Prof. Zhang Y and Dr. Wang H for kindly providing p15A-Cm and E. coli GBdir-gyrA462.

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of ChemistryGeorgia State UniversityAtlantaUSA

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