RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-Negative Bacteria
RecET direct cloning enables obtaining a large DNA region from genome. Here we describe its applications in cloning of polysaccharide gene cluster from gram-negative bacteria. Rapid and exact cloning of polysaccharide gene cluster can be achieved by this method.
Key wordsRecET direct cloning Polysaccharide gene cluster Gram-negative bacteria Genome isolation Restriction enzyme digestion Linear cloning vector Electroporation
This work was supported by NIH grant R01AI083754. Thank Prof. Zhang Y and Dr. Wang H for kindly providing p15A-Cm and E. coli GBdir-gyrA462.
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