Lymphoma pp 35-60 | Cite as

Flow Cytometry for Non-Hodgkin and Hodgkin Lymphomas

  • Emily Glynn
  • Lori Soma
  • David Wu
  • Brent L. Wood
  • Jonathan R. FrommEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1956)


Multiparametric flow cytometry is a powerful diagnostic tool that permits rapid assessment of cellular antigen expression to quickly provide immunophenotypic information suitable for disease classification. This chapter describes a general approach for the identification of abnormal lymphoid populations by flow cytometry, including B, T, NK, and Hodgkin lymphoma cells suitable for the clinical and research environment. Knowledge of the common patterns of antigen expression of normal lymphoid cells is critical to permit identification of abnormal populations at disease presentation and for minimal residual disease assessment. We highlight an overview of procedures for processing and immunophenotyping non-Hodgkin B- and T-cell lymphomas and also describe our strategy for the sensitive and specific diagnosis of classical Hodgkin lymphoma and nodular lymphocyte predominant Hodgkin lymphoma.

Key words

B cells B-cell lymphoma Clonality Flow cytometry Hodgkin lymphoma Light-chain restriction T cells T-cell lymphoma T-cell receptor V-beta repertoire analysis 



The authors thank the medical technologists in the hematopathology laboratory at the University of Washington for their expert technical assistance.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Emily Glynn
    • 1
  • Lori Soma
    • 1
  • David Wu
    • 1
  • Brent L. Wood
    • 1
  • Jonathan R. Fromm
    • 2
    Email author
  1. 1.Department of Laboratory MedicineUniversity of WashingtonSeattleUSA
  2. 2.Department of Laboratory MedicineUniversity of Washington Medical CenterSeattleUSA

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