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Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes

  • Carlos André Ornelas RicartEmail author
  • Rayner Myr Lauterjung Queiroz
  • Agenor de Castro Moreira dos Santos Júnior
  • Sébastien Charneau
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1955)

Abstract

The preparation of subproteome fractions prior to proteome analysis provides both the enrichment of proteins sub-represented in global proteome analysis and information on the cellular localization of the identified proteins. Here we describe protocols for the preparation of Trypanosoma cruzi surface and extracellular and nuclear fractions for further subproteome analysis.

Key words

Proteome Plasma membrane Biotinylation Cell surface shaving Secretome Nucleus 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Carlos André Ornelas Ricart
    • 1
    Email author
  • Rayner Myr Lauterjung Queiroz
    • 2
  • Agenor de Castro Moreira dos Santos Júnior
    • 1
  • Sébastien Charneau
    • 1
  1. 1.Department of Cell Biology, Laboratory of Protein Chemistry and BiochemistryUniversity of BrasiliaBrasíliaBrazil
  2. 2.Department of BiochemistryCambridge Centre for Proteomics, University of CambridgeCambridgeUK

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