CD8+ T Cell Response to Trypanosoma cruzi Antigens during Chronic Chagas Disease

  • Paola LassoEmail author
  • Jose Mateus
  • John Mario González
  • Adriana Cuéllar
  • Concepción Puerta
Part of the Methods in Molecular Biology book series (MIMB, volume 1955)


Flow cytometry is a valuable technique in cellular immunology that allows evaluating effective parameters of the immune response associated with CD8+ T cells. During Chagas disease, infection caused by Trypanosoma cruzi parasite, similar to other intracellular infectious agents, antigen-specific CD8+ T cells are essential for controlling the infection. However, CD8+ T cell response is only partially effective in some chronic Chagas disease patients. Thus, characterization and phenotyping of T. cruzi-specific CD8+ T cells are of great importance during chronic Chagas disease.

Key words

Chagas disease CD8+ T cells Polyfunctionality Inhibitory receptors Phenotype Cytometry 



We thank the patients and healthy volunteers who participated in the study. We thank the following for financial support: COLCIENCIAS (Code 120349326159) and Pontificia Universidad Javeriana (No. 00006233).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Paola Lasso
    • 1
    • 2
    Email author
  • Jose Mateus
    • 1
    • 2
  • John Mario González
    • 3
  • Adriana Cuéllar
    • 1
  • Concepción Puerta
    • 2
  1. 1.Grupo de Inmunobiología y Biología CelularFacultad de Ciencias, Pontificia Universidad JaverianaBogotáColombia
  2. 2.Grupo de Enfermedades InfecciosasFacultad de Ciencias, Pontificia Universidad JaverianaBogotáColombia
  3. 3.Grupo de Ciencias Básicas MédicasFacultad de Medicina, Universidad de los AndesBogotáColombia

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