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Trypanosoma cruzi-Specific T-Cell Responses to Monitor Treatment Efficacy in Chronic Chagas Disease

  • María Cecilia Albareda
  • María Ailén Natale
  • Gonzalo Leandro Cesar
  • Melisa Daiana Castro Eiro
  • María Gabriela Alvarez
  • Susana Adriana Laucella
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1955)

Abstract

Chagas disease is the highest impact parasitic disease in Latin America. In recent years, the use of immune-related biomarkers to predict diagnostic and treatment efficacy or to monitor diseases has been considered a promising tool. Our group has worked for the past 20 years on the characterization of different immunological aspects of the T-cell responses to T. cruzi antigens. We have shown that monitoring of appropriate immunological responses can provide earlier and robust measures of treatment.

The Enzyme-Linked ImmunoSPOT (ELISPOT) assays are powerful tools to evaluate antigen-specific immune responses at the single-cell level. Herein, we describe uses of the ELISPOT assay to determine the T. cruzi-specific T-cell populations in PBMCs from chronic chagasic subjects.

Key words

Chagas disease Trypanosoma cruzi Benznidazole ELISPOT T cells 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • María Cecilia Albareda
    • 1
  • María Ailén Natale
    • 1
  • Gonzalo Leandro Cesar
    • 1
  • Melisa Daiana Castro Eiro
    • 1
  • María Gabriela Alvarez
    • 2
  • Susana Adriana Laucella
    • 1
    • 2
  1. 1.Instituto Nacional de Parasitología Dr. M. Fatala ChabenBuenos AiresArgentina
  2. 2.Hospital Interzonal General de Agudos Eva PerónBuenos AiresArgentina

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