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Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi trans-Sialidase

  • Giannina Carlevaro
  • Andrés B. Lantos
  • Gaspar E. Cánepa
  • María de los Milagros Cámara
  • Martín Somoza
  • Carlos A. Buscaglia
  • Oscar Campetella
  • Juan MucciEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1955)

Abstract

Trypanosoma cruzi, the protozoan agent of Chagas disease, has evolved an innovative metabolic pathway by which protective sialic acid (SA) residues are scavenged from host sialylglycoconjugates and transferred onto parasite surface mucin-like molecules (or surface glycoconjugates from host target cells) by means of a unique trans-sialidase (TS) enzyme. TS-induced changes in the glycoprotein sialylation profile of both parasite and host cells are crucial for the establishment of a persistent T. cruzi infection and for the development of Chagas disease-associated pathogenesis. In this chapter, we describe a novel metabolic labeling method developed in our labs that enables straightforward identification and molecular characterization of SA acceptors of the TS-catalyzed reaction.

Key words

Trypanosoma cruzi Sialic acid trans-Sialidase Sialylglycoconjugates Mucins 

Notes

Acknowledgments

GC and MS hold fellowships from CONICET, and MdlMC, CAB, OC and JM are career investigators from the same Institution. This work was supported by the National Institute of Health R01AI104531 to OC, and Agencia Nacional de Promoción Científica y Tecnológica PICT2012-1815 to JM. The funders had no role in decision to publish or preparation of the manuscript.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Giannina Carlevaro
    • 1
    • 2
  • Andrés B. Lantos
    • 1
    • 2
    • 3
  • Gaspar E. Cánepa
    • 1
    • 2
    • 4
  • María de los Milagros Cámara
    • 1
    • 2
    • 5
  • Martín Somoza
    • 1
    • 2
  • Carlos A. Buscaglia
    • 1
    • 2
  • Oscar Campetella
    • 1
    • 2
  • Juan Mucci
    • 1
    • 2
    Email author
  1. 1.Instituto de Investigaciones BiotecnológicasUniversidad Nacional de San MartínSan MartínArgentina
  2. 2.Consejo Nacional de Investigaciones Científicas y TécnicasBuenos AiresArgentina
  3. 3.Laboratorio Dr. LantosBuenos AiresArgentina
  4. 4.Laboratory of Malaria and Vector ResearchNational Institute of Allergy and Infectious Diseases, National Institutes of HealthRockvilleUSA
  5. 5.Instituto de TecnologíaUniversidad Argentina de la Empresa (UADE)Buenos AiresArgentina

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