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AAV-Mediated Gene Delivery to the Lung

  • Laura P. van Lieshout
  • Jakob M. Domm
  • Sarah K. WoottonEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1950)

Abstract

Adeno-associated virus (AAV) vectors are an efficient method of gene delivery to various tissues including the lung. Mouse models are often used as a preliminary preclinical model in order to advance AAV lung gene therapy vectors. In this chapter we describe an AAV purification protocol using heparin affinity chromatography as well as an intranasal and intratracheal method of delivering AAV vectors to the lungs of mice.

Key words

Adeno-associated virus AAV AAV6.2FF Lung gene therapy Lung delivery Lung gene transfer Heparin column chromatography AAV purification Intranasal instillation 

Notes

Acknowledgments

This work was supported by the Lung Association – Ontario and Cystic Fibrosis Canada.

Supplementary material

Video 1

(MP4 34398 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Laura P. van Lieshout
    • 1
  • Jakob M. Domm
    • 1
  • Sarah K. Wootton
    • 1
    Email author
  1. 1.Department of PathobiologyUniversity of GuelphGuelphCanada

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