AAV-Mediated Gene Delivery to the Lung
Adeno-associated virus (AAV) vectors are an efficient method of gene delivery to various tissues including the lung. Mouse models are often used as a preliminary preclinical model in order to advance AAV lung gene therapy vectors. In this chapter we describe an AAV purification protocol using heparin affinity chromatography as well as an intranasal and intratracheal method of delivering AAV vectors to the lungs of mice.
Key wordsAdeno-associated virus AAV AAV6.2FF Lung gene therapy Lung delivery Lung gene transfer Heparin column chromatography AAV purification Intranasal instillation
This work was supported by the Lung Association – Ontario and Cystic Fibrosis Canada.
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