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Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples

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The Extracellular Matrix

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1952))

Abstract

UDP-sugars are important substrates for the synthesis of various cellular glycans and glycoconjugates, many of which play essential roles in the pathobiology of diseases associated with deranged glucose metabolism, such as cancer and type 2 diabetes. Hence, their analysis from cultured cells and especially from tissue samples can give valuable information. This chapter describes a method for UDP-sugar isolation from various sources utilizing ion-pair solid-phase extraction with graphitized carbon cartridges, and their analysis using anion-exchange high-performance liquid chromatography.

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Author information

Authors and Affiliations

  1. Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland

    Sanna Oikari & Markku I. Tammi

  2. Institute of Dentistry, University of Eastern Finland, Kuopio, Finland

    Sanna Oikari

Authors
  1. Sanna Oikari
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  2. Markku I. Tammi
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Corresponding author

Correspondence to Sanna Oikari .

Editor information

Editors and Affiliations

  1. Department of Medicine and Surgery, University of Insubria, Varese, Italy

    Davide Vigetti

  2. Department of Chemistry, University of Patras, Patras, Greece

    Achilleas D. Theocharis

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Oikari, S., Tammi, M.I. (2019). Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples. In: Vigetti, D., Theocharis, A.D. (eds) The Extracellular Matrix. Methods in Molecular Biology, vol 1952. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9133-4_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9133-4_7

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9132-7

  • Online ISBN: 978-1-4939-9133-4

  • eBook Packages: Springer Protocols

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