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Detection of α-Synuclein in Biological Samples Using Mass Spectrometry

  • Ann Brinkmalm
  • Annika Öhrfelt
  • Payel Bhattacharjee
  • Henrik ZetterbergEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1948)

Abstract

Here we describe a method using mass spectrometry to characterize and quantify immuno-enriched α-synuclein forms from biochemically fractionated brain tissue.

Key words

Parkinson’s disease α-Synuclein Mass spectrometry CSF Brain 

Notes

Acknowledgments

Work in the authors’ laboratories is supported by the Michael J. Fox Foundation (MJFF) for Parkinson’s Research, the Swedish Research Council, the European Research Council, the Knut and Alice Wallenberg Foundation, the Torsten Söderberg Foundation, the Eivind and Elsa K:Son Sylvans Foundation, the Märtha and Gustaf Ågrens Foundation, the Olav Thon Foundation, Swedish State Support for Clinical Research (ALFGBG), Alzheimerfonden, Stiftelsen för Gamla Tjänarinnor, and Frimurarestiftelsen.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ann Brinkmalm
    • 1
  • Annika Öhrfelt
    • 1
  • Payel Bhattacharjee
    • 1
  • Henrik Zetterberg
    • 1
    • 2
    • 3
    • 4
    Email author
  1. 1.Department of Psychiatry and Neurochemistry, Institute of Neuroscience and PhysiologyThe Sahlgrenska Academy at the University of GothenburgMölndalSweden
  2. 2.Clinical Neurochemistry LaboratorySahlgrenska University HospitalMölndalSweden
  3. 3.Department of Molecular NeuroscienceUCL Institute of NeurologyLondonUK
  4. 4.UK Dementia Research Institute at UCLLondonUK

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