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Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assays on GPCRs

  • Sridévi M. Ramanoudjame
  • Lucie Esteoulle
  • Stéphanie Riché
  • Jean-François Margathe
  • Thierry Durroux
  • Iuliia A. Karpenko
  • Dominique BonnetEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1947)

Abstract

Fluorescence techniques represent a powerful tool to investigate dynamic and functional architecture of GPCRs. Thus, fluorescent GPCR ligands have found various applications in cellular imaging, in the development of binding assays as replacements for radioligands in the study of ligand–receptor but also in receptor–receptor interactions at the cell surface or in native tissues. To extend the applicability of these techniques, the design and the synthesis of fluorescent probes are critical steps. As there are numerous peptide receptors in the GPCR family, fluorescent peptide-based probes are of importance. Herein, we present a convenient method to facilitate the solution-phase fluorescent labeling of peptides which is based on the chemoselective acylation of α-hydrazinopeptides. This approach combines the advantages to use commercially available amine-reactive dyes and very mild conditions that are fully compatible with the chemical sensitivity of the dyes. It gives a rapid access to fluorescent peptidic probes compatible with the time-resolved fluorescence resonance energy transfer (TR-FRET) techniques.

Key words

Chemical ligation Chemoselective acylation Hydrazinopeptides Fluorescent probes GPCR TR-FRET 

Notes

Acknowledgements

The development of the method was supported by the Fonds Unique Interministériel (Cell2lead program n° F1005035J/ATFUAA00LB/AAP9), the Centre National de la Recherche Scientifique and the Université de Strasbourg. Sridévi M. Ramanoudjame and Lucie Esteoulle contributed equally to this work.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Sridévi M. Ramanoudjame
    • 1
  • Lucie Esteoulle
    • 1
  • Stéphanie Riché
    • 1
  • Jean-François Margathe
    • 1
  • Thierry Durroux
    • 2
  • Iuliia A. Karpenko
    • 1
  • Dominique Bonnet
    • 1
    Email author
  1. 1.Laboratoire d’Innovation Thérapeutique, Faculté de PharmacieUMR 7200 CNRS/Université de Strasbourg, LabEx MEDALISIllkirchFrance
  2. 2.CNRS UMR 5203Institut de Génomique Fonctionnelle and INSERM U1191 and Université de MontpellierMontpellierFrance

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