A Simple Static Biofilm Assay for Acinetobacter baumannii

  • Indranil BiswasEmail author
  • Joshua Mettlach
Part of the Methods in Molecular Biology book series (MIMB, volume 1946)


Acinetobacter baumannii and other Acinetobacter spp. display biofilm formation on abiotic surfaces. The degree of biofilm formation varies considerably depending on the isolates. While culturing, bacteria tend to attach to abiotic surfaces, and some surfaces perform better than others. Numerous methods are available to study in vitro biofilm formation by bacteria under static growth, and they basically rely upon culturing the bacteria in an ampule followed by staining the bacterial growth attached to the abiotic surface with a suitable dye such as crystal violet to visualize the biofilm. Subsequently, the dye is eluted with a suitable solvent, and optical density is measured to quantify the attached bacterial mass. In this chapter, we provide a basic and simple yet a powerful method to study biofilm formation by A. baumannii grown under static conditions.

Key words

Acinetobacter baumannii Biofilm formation Static biofilm Air-liquid interface 



Work reported in this publication was supported in part by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number P20 GM113117.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Microbiology, Molecular Genetics and ImmunologyUniversity of Kansas Medical CenterKansas CityUSA

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