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Isomeric and Conformational Analysis of Small Drug and Drug-Like Molecules by Ion Mobility-Mass Spectrometry (IM-MS)

  • Shawn T. Phillips
  • James N. Dodds
  • Jody C. May
  • John A. McLeanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1939)

Abstract

This chapter provides a broad overview of ion mobility-mass spectrometry (IM-MS) and its applications in separation science, with a focus on pharmaceutical applications. A general overview of fundamental ion mobility (IM) theory is provided with descriptions of several contemporary instrument platforms which are available commercially (i.e., drift tube and traveling wave IM). Recent applications of IM-MS toward the evaluation of structural isomers are highlighted and placed in the context of both a separation and characterization perspective. We conclude this chapter with a guided reference protocol for obtaining routine IM-MS spectra on a commercially available uniform-field IM-MS.

Key words

Isomers Drugs Conformation Ion mobility spectrometry Ion mobility-mass spectrometry IM-MS 

Notes

Acknowledgments

This work was supported in part using the resources of the Center for Innovative Technology at Vanderbilt University. Financial support for aspects of this research was provided by The National Institutes of Health (NIH Grant R01GM092218) and under Assistance Agreement No. 83573601 awarded by the US Environmental Protection Agency (EPA). This work has not been formally reviewed by the EPA, and the EPA does not endorse any products or commercial services mentioned in this publication. Furthermore, the content is solely the responsibility of the authors and should not be interpreted as representing the official views and policies, either expressed or implied, of the funding agencies and organizations.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Shawn T. Phillips
    • 1
  • James N. Dodds
    • 1
  • Jody C. May
    • 1
  • John A. McLean
    • 1
    Email author
  1. 1.Department of Chemistry, Center for Innovative Technology, Vanderbilt Institute of Chemical Biology, Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt-Ingram Cancer CenterVanderbilt UniversityNashvilleUSA

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