Miniaturized Checkerboard Assays to Measure Antibiotic Interactions
Drugs may have synergistic or antagonistic interactions when combined. Checkerboard assays, where two drugs are combined in many doses, allow sensitive measurement of drug interactions. Here, we describe a protocol to measure the pairwise interactions among three antibiotics, in duplicate, in 5 days, using only two 96-well microplates and standard laboratory equipment.
Key wordsDrug interactions Checkerboard assay Drug synergy