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Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function

  • Andres BarriaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1941)

Abstract

Organotypic slice cultures enable the study of glutamate receptors in an environment closely related to the in vivo situation but with easy access to genetic manipulation of the receptors and regulatory signaling cascades as well as a more precise pharmacological intervention. We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes, and culture medium is allowed to form an interface. This method preserves the functionality and gross architecture of the hippocampus for up to 2 weeks in culture.

Key words

Brain slices Organotypic cultures Glutamate receptors Synaptic transmission Synaptic physiology 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Physiology and BiophysicsUniversity of Washington School of MedicineSeattleUSA

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