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Gene Expression Analysis by Multiplex Single-Cell RT-PCR

  • Ludovic Tricoire
  • Bruno Cauli
  • Bertrand LambolezEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1941)

Abstract

Brain circuit assemblies comprise different cellular subpopulations that exhibit morphological, electrophysiological, and molecular diversity. Here we describe a protocol which, combined with whole-cell patch-clamp recording and morphological reconstruction, allows the transcriptomic analysis of the recorded cell. This protocol provides recipes on how to detect simultaneously the expression of 24 genes/markers at the single-cell level using polymerase chain reaction (PCR), how to design gene-specific probes, and how to validate them. This technique provides multiplexed expression data that cannot be easily obtained by other approaches such as immunological co-labeling.

Key words

mRNA cDNA Cell type Transcriptome Electrophysiology 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ludovic Tricoire
    • 1
  • Bruno Cauli
    • 1
  • Bertrand Lambolez
    • 1
    Email author
  1. 1.Sorbonne Université, UPMC Univ Paris 06 UM119, Centre National de la Recherche Scientifique (CNRS) UMR8246, Institut National de la Santé et de la Recherche Médicale (INSERM) UMRS1130, Neuroscience Paris Seine,Institut de Biologie Paris-SeineParisFrance

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