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Astrocytes pp 117-129 | Cite as

Monitoring Interneuron–Astrocyte Signaling and Its Consequences on Synaptic Transmission

  • Sara Mederos
  • Gertrudis PereaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1938)

Abstract

Whole-cell patch clamp allows the characterization of synaptic transmission in neurons. It is possible to manipulate astrocytic activity and record how these glial cells affect neuronal networks. Here we describe the methodology to monitor the endogenously activation of astrocytes by inhibitory synaptic activity. Afterward, such glial activation will let us study the consequences of interneuron–astrocyte signaling on excitatory neurotransmission at hippocampal synapses.

Key words

Astrocytes Synaptic transmission Whole-cell patch clamp Neuron–glia networks 

Notes

Acknowledgments

This work was supported by PhD fellowship program (MINECO, BES-2014-067594) to S.M; and MINECO grants (BFU2016-75107-P) to G.P.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Neuron-Glia Networks LabInstituto Cajal (CSIC)MadridSpain

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