Astrocytes pp 37-47 | Cite as

Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains

  • Yingbo HeEmail author
  • Natalie Taylor
  • Anindya Bhattacharya
Part of the Methods in Molecular Biology book series (MIMB, volume 1938)


Astrocytes play fundamental roles in development and plasticity of the central nervous system. Dysfunction of astrocytes contributes to most of neurological diseases ranging from neurodegenerative diseases to psychological disorders. To better understand the involvement of astrocytes in both physiological and pathological conditions, and further elucidate their underlying mechanisms, it is critical to establish a reliable isolation and culture method for the cells. In this chapter, we describe the isolation and culture protocols of astrocytes from postnatal and adult mouse brains.

Key words

Astrocytes Isolation Cell culture Postnatal Adult 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yingbo He
    • 1
  • Natalie Taylor
    • 1
  • Anindya Bhattacharya
    • 1
  1. 1.Neuroscience Drug DiscoveryJanssen Research and Development, LLCSan DiegoUSA

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