Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator
Astrocytes are heterogeneous cells of the central nervous system whose uptake of neurotransmitters and neuromodulators can influence synaptic signaling. Any malfunction in this process can lead to serious defects in synaptic transmission found in, for example, neurodegenerative diseases like Alzheimer’s or epilepsy.
Here we describe how to visualize the uptake of an extracellularly located protein by in vitro cultured astrocytes on the example of tissue plasminogen activator, a serine protease tightly involved in long-term potentiation and seizure generation.
Key wordsAstrocytes Tissue plasminogen activator Protein uptake Gliotransmitters Neuromodulators LRP1
We thank the IGSN and the DFG, SPP1757 Fa 159/20-1,2 for providing financial support. We also thank Egor Dzyubenko for valuable discussions and help with establishing the astrocytic cultures and Sandra Lata and David Wegrzyn for performing the “test runs” of the protocol and their valuable feedback.
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