Current Use of Adenovirus Vectors and Their Production Methods

  • Ekramy E. Sayedahmed
  • Rashmi Kumari
  • Suresh K. MittalEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1937)


Various adenovirus (AdV) vector systems have proven to be lucrative options for gene delivery. They can serve as potential vaccine candidates for prevention of several common infectious diseases and hold the promise for gene therapy, especially for cancer. Several AdV vector-based therapies are currently at various stages of clinical trials worldwide, which make an immense interest of both the clinicians and researchers. Since these vectors are easy to manipulate, have broad tropism, and have the capability to yield high titers, this delivery system has a wide range of applications for different clinical settings. This chapter emphasizes on some of the current usages of AdV vectors and their production methods.

Key words

Adenovirus vector Gene delivery system Gene therapy Vector design Vector production Recombinant vaccines 



This work was supported by the Public Health Service grant—AI059374 from the National Institute of Allergy and Infectious Diseases, and the Hatch and Animal Health funds.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ekramy E. Sayedahmed
    • 1
  • Rashmi Kumari
    • 1
  • Suresh K. Mittal
    • 1
    Email author
  1. 1.Department of Comparative Pathobiology, Purdue Institute for Inflammation, Immunology, and Infectious Disease, College of Veterinary MedicinePurdue UniversityWest LafayetteUSA

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