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Localized Intra-Arterial Gene Delivery Using AAV

  • Koji HosakaEmail author
  • Fredric P. Manfredsson
  • Brian L. Hoh
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1937)

Abstract

In vivo gene therapy is a tremendous tool for a wide variety of genetic modifications. However, often a specific and precise local administration of the viral vector is necessary to deliver the genetic payload in vivo. For many animal studies using viral vectors, such as those investigating neurological disorders, the vector is targeted directly into the tissue/organ of interest. On the other hand, in vascular disease research, viral vectors are administered systemically, either via a tail vein injection or through catheter-mediated infusion, which results in off-target transduction of cells and tissues. Targeting cells in the vascular wall without off-target activity, however, requires localized delivery in order to efficiently target cells of the internal vasculature. Here we describe a novel murine in vivo targeted intra-arterial viral vector delivery method, which has been developed in order to be able to perform more intricate studies in cardiovascular disease.

Key words

In vivo transduction Intra-arterial transduction Viral vector AAV Cardiovascular diseases 

Notes

Acknowledgment

This work was supported by NIH R01 grant (5R01NS083673, PI: Hoh).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Koji Hosaka
    • 1
    Email author
  • Fredric P. Manfredsson
    • 2
  • Brian L. Hoh
    • 1
  1. 1.Department of Neurosurgery, College of MedicineUniversity of FloridaGainesvilleUSA
  2. 2.Department of Translational Science and Molecular Medicine, College of Human MedicineMichigan State UniversityEast LansingUSA

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