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Normalization for Single-Cell RNA-Seq Data Analysis

  • Rhonda BacherEmail author
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1935)

Abstract

In this chapter, we describe a robust normalization method for single-cell RNA sequencing data. The procedure, SCnorm, is implemented in R and is part of Bioconductor. Also included in the package are diagnostic functions to visualize normalization performance. This chapter provides an overview of the methodology and provides example work-flows.

Key words

Single-cell RNA-seq Normalization Gene expression Read count High-throughput sequencing 
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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of BiostatisticsUniversity of FloridaGainesvilleUSA

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