Legionella pp 241-247 | Cite as

Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila

  • Tomoko KuboriEmail author
  • Hiroki Nagai
Part of the Methods in Molecular Biology book series (MIMB, volume 1921)


Legionella utilizes specialized protein secretion machinery called the type IV secretion system encoded by dot/icm genes to modulate host cellular systems. We describe here the procedure to isolate the core complex of the Dot/Icm type IV secretion system of L. pneumophila based on detergent lysis of bacteria and ultracentrifugation. The isolated protein complex can be applied for biochemical and transmission electron microscopy analysis.

Key words

Legionella Type IV secretion Core complex Electron microscopy Nanomachine Molecular structure Ultracentrifugation 



This work has been financially supported by MEXT/JSPS KAKENHI Grants 15H01322 (to TK). The protocol presented here has been adapted from Kubori et al. [5].


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Microbiology, Graduate School of MedicineGifu UniversityGifuJapan

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