Bioinformatic Analysis of Small RNA Sequencing Libraries
Bioinformatic analysis of small RNA sequencing libraries consists of transforming a series of small RNA sequencing experiment fastq files into a table containing small RNA sequences and their abundance. This is achieved by cleaning the reads, aligning the cleaned reads to a reference, and parsing the alignment results. In this protocol we present the most common option, and the rationale, for each of these steps.
Key wordsSmall RNA miRNA Adapter Bowtie ShortStack Bioinformatics Sequences
Work in the SDF laboratory was financed by the Mexican National Council of Science and Technology (CONACyT) grants CB-2012-177739 and FC-2015-2/1061.
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