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Functional Analysis of Root microRNAs by a Constitutive Overexpression Approach in a Composite Plant System

  • Damien Formey
  • José Ángel Martín-Rodríguez
  • Georgina HernándezEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1932)

Abstract

Agrobacterium-mediated transformation is a fast and efficient method for genome modification in plants. In this protocol, we apply this technique for the analysis of root microRNA functionality. The induction of hairy roots constitutively overexpressing a given microRNA precursor allows us, in a simple way, to modify the accumulation of specific mature microRNA and analyze the consequence of this alteration on a phenotype of interest. This method generates ready-to-phenotype “composite plants” with untransformed aerial part and microRNA-overexpressing root system, in about 20 days.

Key words

microRNA Overexpression Agrobacterium rhizogenes T-DNA Hairy roots 

Notes

Acknowledgments

The work in our group has been supported by Dirección General de Asuntos del Personal Académico (DGAPA)—UNAM (grants no. PAPIIT IN200816 and IA203218).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Damien Formey
    • 1
  • José Ángel Martín-Rodríguez
    • 1
  • Georgina Hernández
    • 1
    Email author
  1. 1.Centro de Ciencias GenómicasUniversidad Nacional Autónoma de México (UNAM)CuernavacaMexico

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