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Sorghum pp 49-59 | Cite as

Discovery of Sorghum Haploid Induction System

  • Tanveer HussainEmail author
  • Cleve Franks
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1931)

Abstract

Sorghum is the fifth most important cereal grain crop after corn, wheat, rice, and pearl millet in the world. Conventional sorghum breeding relies on multiple generations of self-pollination to achieve the adequate levels of homozygosity for hybrid evaluation, which adds several years and great cost to the breeding process. As in maize, doubled haploid (DH) is the key technology to speed up the breeding process in sorghum. Through 3 years of efforts, two haploid inducer lines, SMHI01 and SMHI02, were discovered by screening 4000 germplasms worldwide. These two inducers have been evaluated in different growth environments and have shown to generate haploids at frequency of 1–2%. The putative haploids produced with these two inducers were evaluated and ploidy was confirmed cytologically and biochemically. The discovery of these inducer lines is the first step toward a revolutionary change in sorghum breeding.

Key words

Haploid Doubled haploid Haploid inducer Sorghum Microspores Pollen Plant regeneration Chromosome doubling 

Notes

Acknowledgments

This study is a collaborative research project between DowDuPont™ and the United Sorghum Checkoff Program. All research was conducted by DowDuPont™ scientists and the research funding was provided by the United Sorghum Checkoff Program.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Corteva Agriscience™, Agriculture Division of DowDuPont™JohnstonUSA

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