Sorghum pp 209-220 | Cite as

Evaluation of Agronomic Performance of β-Carotene Elevated Sorghum in Confined Field Conditions

  • Ping Che
  • Zuo-Yu ZhaoEmail author
  • Mark Hinds
  • Kristen Rinehart
  • Kimberly Glassman
  • Marc Albertsen
Part of the Methods in Molecular Biology book series (MIMB, volume 1931)


To help alleviate vitamin A deficiency in Africa, we have developed nutritionally enhanced sorghum with stabilized high all-trans-β-carotene accumulation. Toward the finalization of this nutritionally enhanced sorghum for food production, confined field trials were conducted to determine the agronomic performance of thirteen independent transgenic events in Iowa and Hawaii. Through these trials, three leading events with no negative impact on agronomic performance were identified. The studies described in this chapter have laid the groundwork for development of the next generation of β-carotene elevated sorghum as a food product.

Key words

Sorghum Agronomic performance Field trial β-carotene elevated β-carotene stability Transgenic Nutritionally enhanced sorghum 



This research was supported by Bill and Melinda Gates Foundation Grand Challenges in Global Health Grant ID-37877 and by The Howard G. Buffett Foundation funding of the Africa Biofortified Sorghum Project. DowDuPont™ provided funding and in-kind donations. This work was done in close collaboration with Dr. Florence Wambugu and her staff at Africa Harvest Biotech Foundation International headquartered in Nairobi, Kenya.


  1. 1.
    WHO (2017) Micronutrient deficiencies. World Health Organization. Accessed 1 May 2018
  2. 2.
    Bouis HE, Saltzman A (2017) Improving nutrition through biofortification: a review of evidence from HarvestPlus, 2003 through 2016. Glob Food Sec 12:49–58CrossRefGoogle Scholar
  3. 3.
    Giuliano G (2017) Provitamin A biofortification of crop plants: a gold rush with many miners. Curr Opin Biotechnol 44:169–180CrossRefGoogle Scholar
  4. 4.
    Report G (2009) Investing in the future—a united call to action on vitamin and mineral deficiencies. Accessed 1 May 2018
  5. 5.
    Duodu KG, Taylor JRN, Belton PS, Hamaker BR (2003) Factors affecting sorghum protein digestibility. J Cereal Sci 38(2):117–131CrossRefGoogle Scholar
  6. 6.
    Shewry PR (2007) Improving the protein content and composition of cereal grain. J Cereal Sci 46(3):239–250CrossRefGoogle Scholar
  7. 7.
    Che P, Zhao ZY, Glassman K, Dolde D, Hu TX, Jones TJ et al (2016) Elevated vitamin E content improves all-trans beta-carotene accumulation and stability in transgenic sorghum. Proc Natl Acad Sci U S A 113:11040–11045CrossRefGoogle Scholar
  8. 8.
    You H, Zhang Y, Zhao ZY, Che P, Albertsen M, Glassman K et al (2015) Quantifying the bioefficacy of β-carotene-biofortified sorghum using a Mongolian gerbil model. FASEB J 29(1). Supplement 605.3Google Scholar
  9. 9.
    Wu E, Lenderts B, Glassman K, Berezowska-Kaniewska M, Christensen H, Asmus T et al (2014) Optimized agrobacterium-mediated sorghum transformation protocol and molecular data of transgenic sorghum plants. In Vitro Cell Dev Biol Plant 50(1):9–18CrossRefGoogle Scholar
  10. 10.
    Shen B, Allen WB, Zheng P, Li C, Glassman K, Ranch J et al (2010) Expression of ZmLEC1 and ZmWRI1 increases seed oil production in maize. Plant Physiol 153:980–987CrossRefGoogle Scholar
  11. 11.
    Ostertagová E, Ostertag O (2013) Methodology and application of one-way ANOVA. Am J Mech Eng 1(7):256–261. Scholar
  12. 12.
    Brown T (2003) Current protocols in molecular biology, Unit 2.9 A southern blotting, published online: 1 May 2001., Copyright © 2003 by John Wiley and SonsGoogle Scholar
  13. 13.
    Tang G, Qin J, Dolnikowski GG, Russell RM, Grusak MA (2009) Golden rice is an effective source of vitamin A. Am J Clin Nutr 89:1–8CrossRefGoogle Scholar
  14. 14.
    Tang G, Hu Y, Yin SA, Wang Y, Dallal GE, Grusak MA et al (2012) B-carotene in Golden Rice is as good as b-carotene in oil at providing vitamin A to children. Am J Clin Nutr 96:658–664CrossRefGoogle Scholar
  15. 15.
    Howe JA, Maziya-Dixon B, Tanumihardjo SA (2009) Cassava with enhanced b-carotene maintains adequate vitamin A status in Mongolian gerbils (Meriones unguiculatus) despite substantial cis-isomer content. Br J Nutr 102:342–349CrossRefGoogle Scholar
  16. 16.
    La Frano MR, Woodhouse LR, Burnett DJ, Burri BJ (2013) Transgenic cassava increases b-carotene and vitamin A concentrations in the TAG-rich plasma layer of American women. Br J Nutr 110:310–320CrossRefGoogle Scholar
  17. 17.
    Li S, Nugroho A, Rocheford T, White WS (2010) Vitamin A equivalence of the b-carotene in b-carotene–transgenic maize porridge consumed by women. Am J Clin Nutr 92:1105–1112CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ping Che
    • 1
  • Zuo-Yu Zhao
    • 1
    Email author
  • Mark Hinds
    • 1
  • Kristen Rinehart
    • 1
  • Kimberly Glassman
    • 1
  • Marc Albertsen
    • 1
  1. 1.Corteva Agriscience™, Agriculture Division of DowDuPont™JohnstonUSA

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